Cloning is the creation of an exact genetic replica of a small segment of DNA, a cell or a whole organism. DNA cloning is also known as recombinant DNA technology, molecular cloning and gene cloning. It is a form of cloning that has been around since the 1970s and the technique is common practice in molecular biology laboratories when multiple copies of genes need to be made for testing for health and forensic purposes. DNA cloning is used to produce many copies of a particular segment of DNA containing one or more genes, to be studied in the laboratory. The DNA fragment of interest from an organism such as a human is incorporated into the ‘plasmid DNA’ of a bacterial cell. A plasmid is a circular self-replicating DNA molecule that is separate from the bacterial DNA. The plasmid containing the genes or DNA of interest is now a piece of ‘recombinant DNA’ made up of human and bacterial DNA. It is then put into a cell that will act as a host: as the host cell is copied over and over again, the recombinant DNA is copied as well. Bacteria are most often the host cells but yeast and mammalian cells can be used too. The end result is multiple identical copies of the same human DNA fragment or gene. Cloning is essential to enable enough copies of a gene or DNA segment to be analysed for human genetic testing to diagnose genetic conditions and enable predictive or presymptomatic genetic testing for genetic conditions in asymptomatic individuals or prenatally. Cloning presents many complex ethical questions. A few to consider are who should have access to the technologies, how will the use of the technologies be monitored, who gets to decide what aspects of cloning are morally acceptable and legal to use and which are not. The simple and lucid presentation of complicated topics in cloning, genetics and stem cell technology would prove to be very useful to students, researchers and teachers.
AUTOR: Whelan, Mark
EDITORA: Auris Reference
DISPONIBILIDADE DO PRODUTO: Sob Encomenda - Até 40 dias ( Importação )